Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome Scientific Reports Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Taking Tylenol? The Glutathione Connection Dr. Green Mom Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA